Sequential separation of immunoglobulin Y and phosvitin from chicken egg yolk without using organic solvents
نویسندگان
چکیده
منابع مشابه
Sequential separation of immunoglobulin Y and phosvitin from chicken egg yolk without using organic solvents.
A study was conducted to develop a simple sequential separation protocol to separate phosvitin and IgY from egg yolk without using organic solvents. Egg yolk was diluted with 2 volumes of distilled water (DW), homogenized, and centrifuged. The precipitant was collected and homogenized with 4 volumes of 10% NaCl (wt/vol) in 0.05 N NaOH solution to extract phosvitin. The pH of the homogenate was ...
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The objective of this study was to develop a new method to separate phosvitin from egg yolk without using organic solvents. Phosvitin was extracted from yolk granules using 10% NaCl or 10% (NH4)2SO4 (final concentration) and then treated with heat to precipitate the lipoproteins from the extracted solution. The optimal pH for the phosvitin extraction from yolk granules was determined, and the i...
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ژورنال
عنوان ژورنال: Poultry Science
سال: 2014
ISSN: 0032-5791
DOI: 10.3382/ps.2014-04093